Anchor line is kind of tricky. Stronger is better, as would be obvious. What is less obvious is stretch. Properly sized anchor line will have some give to it, lessening shock on deck fittings (and people on-board) in swell and chop. The difference between 3/8" and 1/2" in rough water can be surprising.
For a boat your size, I'd say 3/8" twisted nylon is about right (I use 400 ft of that on my 18' RIB.) Marinized rope (as opposed to standard hardware store rope) has a coating that supposedly limits water absorbtion and such - don't know if it really does anything other than increase costs.
For marking, you can sew/weave in colored threads (though I think 5M intervals is a little overkill), or get plastic markers from a chandlery (plastic flags, marked for length, that get slipped into the line between strands.) Personally, I'd think zip-ties would really hurt if your cold hands caught them at the wrong time.
jky
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